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SIDER2 retroposon-mediated mRNA decay in Leishmania is coupled to translation.

Research paper by Hiva H Azizi, Michaela M Müller-McNicoll, Barbara B Papadopoulou

Indexed on: 21 Mar '17Published on: 21 Mar '17Published in: International Journal for Parasitology



Abstract

We previously reported that Short Interspersed Degenerate Retroposons of the SIDER2 subfamily predominantly located within 3' untranslated regions (UTRs) of Leishmania transcripts promote rapid turnover that is initiated by endonucleolytic cleavage. Here, we investigated whether SIDER2-mediated decay is linked to translation. We show that preventing translation initiation by inserting a hairpin structure at the 5'-end of a SIDER2-containing mRNA blocks degradation. Similarly, global inhibition of translation elongation by cycloheximide or termination by puromycin causes stabilization of SIDER2-containing transcripts. Altogether, these findings support that the mechanism of SIDER2-mediated decay is coupled to translation, possibly through the recruitment of decay factors to elongating ribosomes.