Indexed on: 01 Mar '68Published on: 01 Mar '68Published in: Chromatographia
All dansylaminoacids can be separated by 2 twodimensional chromatographies, combined with chromato-electrophoresis on cellulose powder. In all three cases, partition chromatography takes place, in the first dimension, on ammoniumsalts of dansylaminoacids in the solvent toluene/glycol chlorhydrine/pyridine/ammonia 0.8 N (150 ml : 90 ml : 45 ml : 90 ml), +200 mg of primary octyl alcohol. Separation of the ether-soluble compounds is obtained by “salting-out chromatography” in the second dimension: Water/water saturated with ammonium sulphate/sodium dodecylsulphate (700 ml/100 ml/576 mg). As for the dicarboxyl-dansylaminoacids, their separation is obtained, after a second partition chromatography, on a thin layer impregnated with sodium acetate (0.2 M), by means of the solvent isobutanol/acetic acid/water (150/3 to 5/20). Electrophoresis, in the second dimension (diethylamine 0.033 M; sodium chloride 0.02 M), permits the separation of the acid-soluble derivatives. Mixtures of dinitrophenyl-aminoacids and dansylaminoacids can be analyzed simultaneously.