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Séparation de peptides sur couche mince de poudre de cellulose sans liant. 2ème Partie: Séparation par chromatographie à deux dimensions et par chromato-électrophorèse des composants des mélanges de peptides, peptides plus simples et aminoacides qui en dérivent par hydrolyse

Research paper by R. L. Munier, B. Faivre, A. Lebreau

Indexed on: 01 Feb '73Published on: 01 Feb '73Published in: Chromatographia



Abstract

It is shown that two-dimensional chromatography and association of chromatography and electrophoresis on thin layers of binder-free cellulose powder are efficient methods for the separation of the components of mixtures of peptides and their hydrolysis products (lower peptides and amino-acids). These results are obtained using low-voltage electrophoresis (ca. 10 volts/cm), partition chromatography and new solvents (mobile phases). Choice of solution for electrophoresis and of solvent for chromatography has a most important bearing on the quality of separations. By way of example, the efficiency of some new solvents [1] for the separation of the components of mixtures of peptides and aminoacids is shown.