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Separation and subfractionation of small numbers of cells (∼106) by countercurrent distribution in dextran-poly(ethylene glycol) aqueous-phase systems

Research paper by Harry Walter, Eugene J. Krob

Indexed on: 01 Dec '84Published on: 01 Dec '84Published in: Cell Biochemistry and Biophysics



Abstract

Separation and subfractionation of cells on the basis of subtle differences in surface properties by partitioning in dextran-poly(ethylene glycol) aqueous phase systems is an established method. We report here that the incorporation of fetal bovine serum into such systems permits countercurrent distribution of small quantities of cells (∼106). In the absence of serum such small quantities of cells are lost (probably by adherence) and cannot be recovered after countercurrent distribution.