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Sensitive monitoring of taurine biomarker in unprocessed human plasma samples using a novel nanocomposite based on poly(aspartic acid) functionalized by graphene quantum dots.

Research paper by Nasrin N Shadjou, Salar S Alizadeh, Mohammad M Hasanzadeh

Indexed on: 26 Jun '18Published on: 26 Jun '18Published in: Journal of Molecular Recognition



Abstract

The rapid and accurate determination of the level of taurine biomarker in various tissues and body fluids can be of great interest in the early diagnosis of several important pathologies and diseases. For the first time, this study reports on the electropolymerization of a low toxic and biocompatible nanocomposite "poly(aspartic acid)-graphene quantum dots (GQDs)" as a novel strategy for surface modification of glassy carbon electrode and preparation a new interface for measurement of taurine. Electrochemical deposition, as a well-controlled synthesis procedure, has been used for subsequently layer-by-layer preparation of GQDs nanostructures on poly(aspartic acid) using cyclic voltammetry techniques in the regime of -1.5 to 2 V. The field emission scanning electron microscopy indicated immobilization of uniformly GQDs onto poly(aspartic acid) film. The modified electrode appeared as an effective electroactivity for detection of taurine biomarker using cyclic voltammetry, chronoamperometry, and differential pulse voltammetry. Enhancement of peak currents is ascribed to the fast heterogeneous electron transfer kinetics that arise from the synergistic coupling between the excellent properties of poly(aspartic acid) as semiconducting polymer, GQDs as high density of edge plane sites, and subtle electronic characteristics to chemical modification. Under the optimized analysis conditions, the prepared sensor for detection of taurine showed a low limit of quantification 0.001mM. Finally, the resulting prepared sensor allow the quantification of these biomarkers directly in biological samples without need of derivatization schemes or sample pretreatment. Copyright © 2018 John Wiley & Sons, Ltd.

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