Self-Assembling Protein-Polymer Bioconjugates for Surfaces with Antifouling Features and Low Non-Specific Binding.

Research paper by Yingying Y Liu, Tarja K TK Nevanen, Arja A Paananen, Kristian K Kempe, Paul P Wilson, Leena-Sisko LS Johansson, Jussi Joonas JJ Joensuu, Markus B MB Linder, David M DM Haddleton, Roberto R Milani

Indexed on: 20 Dec '18Published on: 20 Dec '18Published in: ACS Applied Materials & Interfaces


A new method is demonstrated for preparing antifouling and low non-specific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pre-treatments. The surface-active protein hydrophobin was covalently modified with a controlled radical polymerization initiator and allowed to self-assemble as a monolayer on hydrophobic surfaces, followed by the preparation of antifouling surfaces by Cu(0)-mediated living radical polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA) performed in situ. By taking advantage of hydrophobins to achieve at the same time the immobilization of protein A, this approach al-lowed to prepare surfaces for IgG binding featuring greatly reduced non-specific adsorption. The success of the surface modification strategy was investigated by contact angle, XPS and AFM characterization, while the antifouling perfor-mance and the reduction of non-specific binding were confirmed by QCM-D measurements.