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Screening of the potentially active compounds from Polygonatum sibiricum using RAW264.7 cellular membranes coated magnetic beads fishing followed by HPLC analysis.

Research paper by Qing-Ling QL Qi, Ya-Ming YM Yu, Cheng C Tang

Indexed on: 28 Nov '19Published on: 27 Nov '19Published in: Biomedical Chromatography



Abstract

Target biomolecule-immobilized magnetic beads (MBs) could be used as a powerful tool for screening active compounds present in natural products. Low damage rates of the target proteins, associated with the availability of diverse automated online approaches for analysis, make it a valuable tool for affinity studies. RAW264.7 cells (a kind of murine macrophage cell line) were used in this study. These cellular membranes were immobilized onto the surface of MBs and were used for screening the active compounds of Polygonatum sibiricum. Combining this technique with HPLC led to the identification of an active compound and its biological activity was confirmed. This is the first report establishing the use of RAW264.7 cellular membranes coated MBs fishing followed by HPLC analysis for screening active compounds from natural products. This article is protected by copyright. All rights reserved.