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Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair.

Research paper by Melike M Cağlayan, Vinod K VK Batra, Akira A Sassa, Rajendra R Prasad, Samuel H SH Wilson

Indexed on: 30 Apr '14Published on: 30 Apr '14Published in: Nature Structural and Molecular Biology



Abstract

DNA polymerase β (pol β) lyase removal of 5'-deoxyribose phosphate (5'-dRP) from base excision repair (BER) intermediates is critical in mammalian BER involving the abasic site. We found that pol β also removes 5'-adenylated dRP from BER intermediates after abortive ligation. The crystal structure of a human pol β-DNA complex showed the 5'-AMP-dRP group positioned in the lyase active site. Pol β expression rescued methyl methanesulfonate sensitivity in aprataxin (hnt3)- and FEN1 (rad27)-deficient yeast.