Indexed on: 24 Apr '16Published on: 24 Apr '16Published in: Biomedical Chromatography
This study was conducted to characterize the residual level and perform a risk assessment of buprofezin formulated as an emulsifiable concentrate (EC), wettable powder (WP), and suspension concentrate (SC) over various treatment schedules in plum, i.e., Prunus domestica. The samples were extracted with an AOAC quick, easy, cheap, effective, rugged, and safe "QuEChERS" method after major modifications. As intrinsic interferences were observed in blank plum samples following dispersive-solid phase extraction (d-SPE consisting of primary secondary amine (PSA) and C18 sorbents), amino cartridges were used for solid-phase extraction (SPE). Analysis was carried out using LC with diode array detection (DAD) and confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method showed excellent linearity with determination coefficient (R(2) = 1) and satisfactory recoveries (at two spiking level; 0.5 and 2.5 mg/kg) between 90.98-94.74% with relative standard deviation (RSD) ≤ 8%. The limit of quantification (LOQ = 0.05 mg/kg) was considerably lower than the maximum residue limit (MRL; 2 mg/kg) set by the Codex Alimentarius. Absolute residue levels for EC were highest, perhaps due to the dilution rate and adjuvant. Notably, all formulation residues were lower than the MRL, and safety data proved that the fruits are safe for consumers.