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Recombinant-protein production in insect cells utilizing a hollow-fibre bioreactor.

Research paper by Sabine C SC Baxter, Dominick D Panarello, Sandya S Ajith, Madhushree M Bhattacharya, Irwin M IM Chaiken

Indexed on: 29 Jul '06Published on: 29 Jul '06Published in: Biotechnology and Applied Biochemistry



Abstract

Here, we demonstrate for the first time that the hollow-fibre bioreactor is an excellent tool for the production of Drosophila-expressed recombinant proteins. Using the example of the soluble extracellular portion of the human IL-5 (interleukin 5) receptor alpha expression in S2 (Schneider's Drosophila melanogaster cell line 2) cells, we found that it is possible to produce multi-milligram amounts of functional recombinant protein continuously for several months on a laboratory scale with minimal maintenance requirements. The insect cells grow to high density and express concentrated functional recombinant protein in a small volume, simplifying and economizing downstream purification.