Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo

Research paper by Anyanee Kamkaew, Haiyan Sun, Christopher G. England, Liang Cheng, Zhuang Liu, Weibo Cai

Indexed on: 10 May '16Published on: 28 Apr '16Published in: Chemical Communications


A small luciferase protein (Nluc) was conjugated to QDs as a bioluminescence resonance energy transfer (BRET) pair. The conjugate showed 76% BRET efficiency and lymph node mapping was successfully performed. The cRGD peptide was conjugated to QD-Nluc for tumor targeting. The self-illuminating QD-Nluc showed excellent energy transfer in a living system and offered an optimal tumor-to-background ratio (>85).

Graphical abstract 10.1039/C6CC02764D.gif