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Quantitative RP-HPLC Determination of Some Aldehydes and Hydroxyaldehydes as Their 2,4-Dinitrophenylhydrazone Derivatives.

Research paper by E E Koivusalmi, E E Haatainen, A A Root

Indexed on: 01 Jan '99Published on: 01 Jan '99Published in: Analytical Chemistry



Abstract

A high-performance liquid chromatographic (HPLC) method is described for the quantitative determination of some aliphatic aldehydes and β-hydroxyaldehydes as their 2,4-dinitrophenylhydrazone derivatives. A method is described for the preparation of derivatives for those β-hydroxyaldehydes where no reference compounds of known purity are available. The detection limit of the method was 4.3-21.0 μg/L, depending on the aldehyde.