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Quantitative proteomics links metabolic pathways to specific developmental stages of the plant pathogenic oomycete Phytophthora capsici.

Research paper by Zhili Z Pang, Vaibhav V Srivastava, Xili X Liu, Vincent V Bulone

Indexed on: 29 Mar '16Published on: 29 Mar '16Published in: Molecular Plant Pathology



Abstract

The oomycete Phytophthora capsici is a plant pathogen responsible for important losses to vegetable production worldwide. Its asexual reproduction plays an important role in the rapid propagation and spread of the disease in the field. A global proteomics study was conducted to compare two key asexual life stages of P. capsici, i.e., the mycelium and cysts, to identify stage-specific biochemical processes. A total of 1200 proteins were identified using qualitative and quantitative mass spectrometry. The transcript abundance of some of the enriched proteins was also analyzed by quantitative real-time PCR. Seventy three proteins exhibited different levels of abundance between the mycelium and cysts. The proteins enriched in the mycelium are mainly associated with glycolysis, the TCA cycle and the pentose phosphate pathway, providing the energy required for the biosynthesis of cellular building blocks and hyphal growth. In contrast, the proteins that are predominant in cysts are essentially involved in fatty acid degradation, suggesting that the early infection stage of the pathogen relies primarily on fatty acid degradation for energy production. The data provide a better understanding of P. capsici biology and suggest potential metabolic targets at the two different developmental stages for disease control. This article is protected by copyright. All rights reserved.