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Quantitative methods for the analysis of CFTR transcripts/splicing variants.

Research paper by Margarida D MD Amaral, Luka A LA Clarke, Anabela S AS Ramalho, Sebastian S Beck, Fiona F Broackes-Carter, Rebecca R Rowntree, Nathalie N Mouchel, Sarah H SH Williams, Ann A Harris, Maria M Tzetis, Bernhard B Steiner, Javier J Sanz, Sabina S Gallati, Malka M Nissim-Rafinifa, Batsheva B Kerem, et al.

Indexed on: 07 Oct '04Published on: 07 Oct '04Published in: Journal of Cystic Fibrosis



Abstract

In cystic fibrosis (CF), transcript analysis and quantification are important for diagnosis, prognosis and also as surrogate markers for some therapies including gene therapy. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods are evolving towards reliable quantification. Various protocols for the quantitative analysis of CFTR transcripts (including those resulting from splicing variants) are described and discussed here.