Quantitative methods for the analysis of CFTR transcripts/splicing variants.

Research paper by Margarida D MD Amaral, Luka A LA Clarke, Anabela S AS Ramalho, Sebastian S Beck, Fiona F Broackes-Carter, Rebecca R Rowntree, Nathalie N Mouchel, Sarah H SH Williams, Ann A Harris, Maria M Tzetis, Bernhard B Steiner, Javier J Sanz, Sabina S Gallati, Malka M Nissim-Rafinifa, Batsheva B Kerem, et al.

Indexed on: 07 Oct '04Published on: 07 Oct '04Published in: Journal of Cystic Fibrosis

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Abstract

In cystic fibrosis (CF), transcript analysis and quantification are important for diagnosis, prognosis and also as surrogate markers for some therapies including gene therapy. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods are evolving towards reliable quantification. Various protocols for the quantitative analysis of CFTR transcripts (including those resulting from splicing variants) are described and discussed here.