Pulp response to dentine adhesives: A study on mature human pulps.

Research paper by Pantelis P Kouros, Eugenia E Koliniotou-Koumpia, Elisabeth E Koulaouzidou, Maria M Helvatjoglu-Antoniades, Dimitrios D Tziafas

Indexed on: 27 Jun '14Published on: 27 Jun '14Published in: European journal of dentistry


To clinically investigate the antibacterial effects of a commercially available self-etch 12-methacryloyloxy- dodecylpyridinium bromide (MDPB)-containing adhesive system in comparison with its respective non-MDPB-containing adhesive and to evaluate the pulp responses when in use on human teeth.Sixty-two viable human teeth scheduled for extraction were used. Class V cavities were prepared on the buccal surfaces of the teeth and filled with the tested materials (Protect Bond/Clearfil AP-X, SE Bond/Clearfil AP-X and Dycal/Ketac Fill Plus) as a control group, according to manufacturer's recommendations. Randomly divided to two groups teeth remained intra-orally for 4 and 8 weeks. After extraction, teeth were decalcified, sectioned and stained using the Mayer's hematoxylin and eosin, and modified Brown-Brenn's technique. Pulp responses were evaluated microscopically under a microscope and remaining dentine thickness measured under a stereomicroscope.No statistically significant differences regarding pulp inflammation or bacterial infiltration were found either for the materials tested or for periods of post-operative evaluation.The results suggested that for a short period of evaluation there are no quantitative differences, regardless to restoring material used.