Protective effects of phenolic fraction of blue honeysuckle fruits against UVA-induced damage to human keratinocytes.

Research paper by Alena A Svobodová, Jana J Rambousková, Daniela D Walterová, Jitka J Vostálová

Indexed on: 12 Apr '08Published on: 12 Apr '08Published in: Archives of Dermatological Research


In this study, the UVA (photo)protective activity of the phenolic fraction of L. caerulea fruits (PFLC) was assessed in human keratinocytes HaCaT. The keratinocytes were pre- or post-treated with PFLC (1-250 mg/l) and exposed to UVA irradiation (10-30 J/cm(2)). The results showed that both pre- and post-treatment with PFLC significantly suppressed UVA-induced ROS production, which was also revealed as a decrease in intracellular lipid peroxidation and elevation of reduced glutathione. Protection was concentration-dependent with a maximum at 50 mg/l. These results suggest that PFLC attenuates UVA-induced oxidative stress by reduction of ROS generation and ROS-mediated damage. For this reason, PFLC has potentially skin-protective functions against the deleterious effects of sunlight.