Production of recombinant proteins in Escherichia coli.

Research paper by E R ER LaVallie

Indexed on: 23 Apr '08Published on: 23 Apr '08Published in: Current protocols in protein science / editorial board, John E. Coligan ... [et al.]


E. coli is the expression system of choice and a substantial body of literature has accumulated on the successful expression of foreign genes in this host. Several problems with protein expression in E. coli have been encountered, and many have been ultimately solved. This unit describes methods that have been developed for production of recombinant proteins in E. coli and potential pitfalls that may be encountered.