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Production of 2-O-α-d-glucopyranosyl l-ascorbic acid using cyclodextrin glucanotransferase from Paenibacillus sp.

Research paper by Hong-Ki Jun, Kyung-Mi Bae, Sung-Koo Kim

Indexed on: 01 Nov '01Published on: 01 Nov '01Published in: Biotechnology Letters



Abstract

Transglycosylation to produce a 2-O-α-d-glucopyranosyl l-ascorbic acid (AA-2G) was studied using cyclodextrin glucanotransferase (CGTase) from Paenibacillus sp. A series of maltooligosaccharides substituted 2-O-derivatives of l-ascorbic acid (AA) were analyzed by HPLC. The maltooligosaccharides were hydrolyzed by glucoamylase to give AA-2G. CGTase also produced AA-2G using dextrin as a glycosyl donor and AA as an acceptor. CGTase utilized α-, β-, and γ-CDs, amylose, soluble starch and corn starch as glycosyl donors but not glucose.