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Preparation of plant DNA for separation by pulsed field gel electrophoresis.

Research paper by K M KM Devos, D D Vercruysse-Dewitte

Indexed on: 01 Apr '89Published on: 01 Apr '89Published in: ELECTROPHORESIS



Abstract

A method was developed for the preparation of completely intact plant DNA embedded in agarose, and suitable for restriction enzyme digestion. Digestion with restriction enzyme was carried out according to modified protocols of Anand and Kenwrick et al. The new method of DNA isolation allows the separation of high molecular weight plant DNA by pulsed field gel electrophoresis.