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Preparation of monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for ornidazole detection

Research paper by Liang Zhao, Jiaying Li, Ye Li, Tiangang Wang, Xiaolu Jin, Kai Wang, Ebeydulla Rahman, Yuan Xing, Baoping Ji, Feng Zhou

Indexed on: 22 Mar '17Published on: 22 Feb '17Published in: Food Chemistry



Abstract

A monoclonal antibody (mAb) and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ornidazole (ONZ) detection were developed. ONZ was conjugated with cationic bovine serum albumin as a hapten to generate the artificial immunogens and coating antigens. BALB/c mice were immunized, and mAbs were obtained. The competitive inhibition curve of ic-ELISA was y = 0.0438x2 − 0.2101x + 0.2925, with R2 = 0.9941. The 50% inhibition concentration, the limit of detection, and limit of quality for ONZ were 0.15, 0.01, and 0.05 µg/kg, respectively. The cross-reactivity of the mAbs to secnidazole was 0.33%. The recoveries were from 89.18% to 101.63% and the coefficient of variation was less than 7.15% in chicken, chicken liver, and honey samples, all of which had ONZ concentrations of 0.05 and 0.1 μg/kg. Results showed that the ic-ELISA based on mAb could be used for the rapid detection for ONZ.

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