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Preparation of cucumber seed peptide-calcium chelate by liquid state fermentation and its characterization

Research paper by Xu Wang, Ang Gao, Yue Chen, Xiaoyuan Zhang, Shuhong Li, Ye Chen

Indexed on: 23 Mar '17Published on: 27 Feb '17Published in: Food Chemistry



Abstract

A specific cucumber seed peptide (CSP) was successfully produced by liquid state fermentation with B. subtilis HYT. Then the CSP was divided into four kind molecular weight components (CSP, untreated; CSP1, MW > 10 kDa; CSP2, 6 kDa < MW < 10 kDa; CSP3, MW < 6 kDa) by ultrafiltration. Among them, the CSP3 displayed the most excellent ability to chelate with calcium (CSP3-Ca). SEM, EDS, ultra-violet spectroscopy, FTIR and Fluorescence spectroscopy were applied to distinguish the characterizations between CSP3 and CSP3-Ca chelate. The results suggested that the apparent characteristics and internal structure of CSP3 were both changed. There were abundant mental-binding sites presented in CSP3, and the <img border="0" alt="single bond" src="http://cdn.els-cdn.com/sd/entities/sbnd" class="glyphImg">COOH, <img border="0" alt="single bond" src="http://cdn.els-cdn.com/sd/entities/sbnd" class="glyphImg">OH, <img border="0" alt="single bond" src="http://cdn.els-cdn.com/sd/entities/sbnd" class="glyphImg">NH2 as well <img border="0" alt="single bond" src="http://cdn.els-cdn.com/sd/entities/sbnd" class="glyphImg">CO<img border="0" alt="single bond" src="http://cdn.els-cdn.com/sd/entities/sbnd" class="glyphImg">NH<img border="0" alt="single bond" src="http://cdn.els-cdn.com/sd/entities/sbnd" class="glyphImg"> might participate in the coordination with Ca2+ to form the CSP3-Ca chelate. Meanwhile, the finding showed that cucumber seeds peptide could chelate with calcium and play the role of novel carries for calcium supplement.

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Figure 10.1016/j.foodchem.2017.02.121.1.jpg
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