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Preliminary studies of micropropagation of Hopea odorata, a dipterocarp tree

Research paper by E. S. Scott, A. N. Rao, C. S. Loh

Indexed on: 01 May '95Published on: 01 May '95Published in: Plant Cell, Tissue and Organ Culture (PCTOC)



Abstract

Plantlet development from in vitro cultures of Hopea odorato Roxb. is described. Embryos excised from seeds and cultured on Gamborg's B5 or modified Murashige and Skoog (MS) medium with benzyladenine (BA, 2.2–22.2 μM) produced axillary shoots at cotyledonary and/or stem nodes. Shoot production was greatest in germinated embryos on modified MS medium with 8.9 μM BA. Excised axillary shoots formed few buds when cultured on medium with BA and limited root development occurred on Woody Plant Medium with naphthaleneacetic acid. Nodal explants from aseptically grown plantlets sprouted axillary shoots in modified MS medium with BA.