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Platinum complexes for multi-parametric assays using microarray systems.

Research paper by María M González, Raquel R Bartolomé, Sergio S Matarraz, Emilio E Rodríguez-Fernández, Juan L JL Manzano, Martín M Pérez-Andrés, Alberto A Orfao, Manuel M Fuentes, Julio J JJ Criado

Indexed on: 25 Nov '11Published on: 25 Nov '11Published in: Journal of Inorganic Biochemistry



Abstract

Here, we present a two novel fluorescent dyes ethylenediaminechlorocholylglycinateplatinum(II), [Pt(CG)Cl(en)] complex 1 and bisursodeoxycholate(ethylenediamine)platinum(II), [Pt(UDC)(2)(en)] complex 2 based on well-known cis-platin chemistry. These platinum complexes contain cholylglycinate (CG) and ursodeoxycholate (UDC) as ligands. These compounds enable qualitative detection of double-helix DNA and quantitative detection (from pg to μg). These novel compounds have absorption and emission spectra in a difference range as the common ones (for example: cyanine dyes such as Cy3, Cy5, Cy7,…); therefore, it could allow the multi-parametric detection of DNA arrays, incrementing the capacity of experimental performance per one single array. As a consequence, it will increase the amount of data info obtained per chip. The combination of the intrinsic property of this compounds with the optical properties in different fluorescence channels, can allow introducing a new molecule with a wide range of possible applications in DNA arrays.

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