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Peptide fragment of respiratory syncytial virus protein G, immunogenic agent, pharmaceutical composition containing it and preparation method

Imported: 24 Feb '17 | Published: 09 Sep '03

Hans Binz, Thien Ngoc N'Guyen, Thierry Baussant, Michel Trudel

USPTO - Utility Patents

Abstract

A polypeptide useful as an immunogen element and characterized in that it is carried on the peptide sequence between amino acid residues 130-230 of the G protein sequence of the human respiratory syncytial virus of sub-groups A and B, or of the bovine respiratory syncytial virus, or on a sequence at least 80% homologous thereto. An immunogenic agent or pharmaceutical composition containing said polypeptide, and methods for preparing and using the same, are also disclosed.

Description

In these examples, reference will be made to the following figures:

FIG.

1: intensity of the immune response induced against G1A in different forms,

FIG.

2: kinetics of the immune response induced against G1A presented in different forms,

FIG.

3: kinetics of the immune response induced against the carrier alone,

FIG.

4: cloning strategy by genetic amplification of p40.

Claims

1. An immunogenic agent comprising a polypeptide carried by the peptide sequence between the amino acid residues 130 and 230 of the sequence of the G protein of human respiratory syncytial virus of subgroup A and of subgroup B, or of bovine respiratory syncytial virus selected from the group consisting of peptides with sequences SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 51 coupled to a carrier protein.

2. An immunogenic agent of claim 1 wherein the carrier protein is an immune adjuvant protein.

3. An immunogenic agent of claim 1 wherein the carrier protein is an OmpA protein.

4. An immunogenic agent of claim 1 wherein the polypeptide is conjugated to the carrier protein by a linking protein.

5. An immunogenic agent of claim 4 wherein the linking protein is the human serum albumin receptor.

6. An immunogenic agent of claim 1 wherein the coupling is a covalent coupling.

7. An immunogenic agent of claim 1 wherein the agent is obtained by a biological route.

8. An immunogenic agent of claim 1, wherein the carrier protein is an OmpA protein of a bacterium of the genus Klebsiella.

9. An immunogenic agent of claim 1, wherein the carrier protein is an OmpA protein of a bacterium of

Klebsiella pneumoniae.

10. An immunogenic agent of claim 1, wherein the carrier protein is the protein p40 having the sequence SEQ ID No. 13 of

Klebsiella pneumoniae or a protein having 80% homology with the protein p40.

11. An immunogenic agent of claim 1, wherein the carrier protein is a protein comprising the SEQ ID No. 13.

12. An immunogenic agent of claim 1, wherein the carrier protein is an OmpA of the external membrane of a bacterium of a genus Klebsiella, in the form of a soluble conjugate.