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Overview of protein purification and characterization.

Research paper by R K RK Scopes

Indexed on: 23 Apr '08Published on: 23 Apr '08Published in: Current protocols in protein science / editorial board, John E. Coligan ... [et al.]



Abstract

This insightful overview by one of the most respected names in protein research discusses a broad array of the aspects involved with protein purification, including historical background, determining the purpose for purifying a particular protein, and actual methods and recommendations for purification procedures. Also covered are methods of analysis and detection, and techniques for characterizing a purified protein. Finally, a useful discussion of the equipment needed in a protein purification lab is provided.