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Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin.

Research paper by Hanzhang H Dong, Gaogao G Liu, Biao B Jiang, Jiubing J Guo, Guoquan G Tao, Wei W Yiu, Jingsong J Zhou, Guoxin G Li

Indexed on: 09 Oct '14Published on: 09 Oct '14Published in: Oncology letters



Abstract

The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression levels of Survivin mRNA and protein in the SGC7901/CDDP and SGC7901 cells were examined by reverse transcription polymerase chain reaction and western blotting respectively. The results revealed morphological differences between the SGC7901 and SGC7901/CDDP cells. The expression levels of Survivin mRNA and protein were significantly higher in the SGC7901/CDDP cells than in the SGC7901 cells. Therefore, high expression levels of the Survivin gene may explain SGC7901 cell resistance to CDDP.