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Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis.

Research paper by Daniel C DC Propheter, Lara K LK Mahal

Indexed on: 21 May '11Published on: 21 May '11Published in: Molecular BioSystems



Abstract

Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.