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Optimization of different doses of growth regulators for in vitro regeneration of Echinochloa frumentacea Roxb. from caryopsis

Research paper by Sweta Dosad, Harvinder Singh Chawla

Indexed on: 23 Oct '15Published on: 23 Oct '15Published in: Indian Journal of Plant Physiology



Abstract

Plants were successfully regenerated in vitro from caryopsis of Echnochloa frumentacea Roxb. cv. PRJ 1 on MS medium supplemented with various concentrations of 2, 4-D (1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0 mg l−1) alone or in combination with kinetin (0.2 and 0.5 mg l−1). Highest callus induction frequency of 41.1 % was recorded on callusing media supplemented with 3.5 mg l−1 2, 4-D alone. Green nodular calli were transferred onto the MS media containing different concentrations and combinations of NAA, kinetin, BAP and GA3. Highest shoot regeneration response of 84.6 % and 17.5 shoots per callus were obtained with callus cultured on NAA containing medium. Shoots that were induced on NAA or 1 mg l−1 kinetin containing medium also showed simultaneous induction of healthy root system, while, shoots induced on rest of the regeneration media lacked well organized root system. Shoots from these media were kept on rooting medium supplemented with different levels and combinations of IBA and NAA. These shoots were rooted on MS medium supplemented with IBA (0.5 or 1.0 mg l−1), NAA (1 mg l−1) and IBA + NAA (0.5 mg l−1 each). Rooting media containing 1 mg l−1 NAA showed best rooting response, inducing 7.5 healthy and long roots per shoot. Regenerated plants were transferred to pots containing sterilized soil: sand: vermiculite: FYM (2:2:2:1) and were irrigated with Hoagland solution. Regenerated plants grew well and were successfully established in soil.