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Optimization of agitation for production of swainsonine from Metarhizium anisopliae in stirred tank and airlift reactors

Research paper by C. Tamerler, T. Keshavarz

Indexed on: 01 Jun '99Published on: 01 Jun '99Published in: Biotechnology Letters



Abstract

The optimal agitation rate for production of swainsonine from Metarhizium anisopliae grown in batch stirred tank reactors (2 to 20 l) was 400 rpm with a mixed hyphal and pelleted morphology where the specific swainsonine production rate was 9×10−2 mg g−1 cell dry wt h from 87 to 142 h. Culture of the fungus in a 6-l airlift reactor produced ‘loose’ pellets and the production of swainsonine started at least 24 h earlier than in the stirred tank reactor. The final yield (5.9 mg swainsonine g−1 cell dry wt) after 168 h in the airlift reactor was 18% less than those obtained in the stirred tank reactor with an agitation rate of 400 rpm.