Online concentration of aristolochic acid I and II in Chinese medicine preparations by micellar electrokinetic chromatography.

Research paper by Shenhua S Shi, Wei W Li, Yiping Y Liao, Zongwei Z Cai, Huwei H Liu

Indexed on: 04 Sep '07Published on: 04 Sep '07Published in: Journal of Chromatography A


In this study, an online concentration method in micellar electrokinetic chromatography (MEKC) applying field-enhanced sample injection (FESI) mode was developed for the detection of aristolochic acids (AAs) in Chinese medicine preparations. AA-I and AA-II were baseline separated with high separation efficiency, and 100-fold enhancement of the detection sensitivity was achieved compared with those obtained from normal capillary zone electrophoresis (CZE) or simple MEKC method. The proposed method was successfully applied for the determination of AAs in Chinese medicine preparations.