Olfactory epithelium grafts in the cerebral cortex: an immunohistochemical analysis.

Research paper by E H EH Holbrook, L J LJ DiNardo, R M RM Costanzo

Indexed on: 22 Jan '02Published on: 22 Jan '02Published in: The Laryngoscope


To develop an alternative model for studying the regenerative capacity of olfactory neurons.An immunohistochemical analysis of mouse olfactory epithelium transplanted to the cerebral cortex.Strips of olfactory epithelium removed from donor mice at postnatal day 5 to day 20 were inserted into the parietal cortex of adult mice. Recipient animals were allowed to survive for 25 to 120 days and then perfused with 4% paraformaldehyde 1 hour after bromodeoxyuridine injection. The brains were processed, and frozen sections were obtained. Sections through transplant tissue were analyzed using immunohistochemistry and compared with normal olfactory epithelium.Graft survival approached 85% with mature olfactory neurons detected in 35% of the transplants stained for olfactory marker protein. Transplant epithelium resembled normal olfactory epithelium containing mature olfactory neurons and axon bundles.Studies of olfactory neuron regeneration have been limited by the inability to produce cultures with long-term viability. Olfactory epithelial grafts to the cerebral cortex provide an alternative approach to the study of olfactory neuron regeneration.