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Nouvelles méthodes et applications en chromatographie et en électrophorèse en couche mince

Research paper by Roger L. Munier

Indexed on: 01 Jan '68Published on: 01 Jan '68Published in: Analytical and Bioanalytical Chemistry



Abstract

After a short introduction on development of chromatographic methods and on importance of thin-layer chromatography for separation of hydrosoluble prodcuts, apparatus for thin-layer chromatography and thin-layer electrophoresis are described. New chromatographic processes are presented: chromatography with double irrigation under definite atmosphere, use of several analytical processes on a same plate, tridirectional chromato-electrophoresis, chromato-electrophoresis using a solvent containing volatile buffer mixture, chromatography using one part of a buffer mixture in solvent with other part of the buffer mixture included in the thin-layer. Applications of these processes to separation of amino-acids, dinitrophenylamino-acids (identification and quantitative determination) and of peptides are described.