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New cyclomaltodextrin glucan transferases produced by bacillus macerans

Research paper by V. A. Abelyan, K. B. Afyan, L. S. Manukyan

Indexed on: 01 Jul '00Published on: 01 Jul '00Published in: Applied Biochemistry and Microbiology



Abstract

—For the first time, microorganisms producing cyclomaltodextrin glucantransferaseglucan transferases (CGT, EC 2.4.1.19) were isolated from soil samples of various ecogeographical regions. These microorganisms were identified asBacillus macerans. The enzymes were purified by affinity chromatography on an α-cyclodextrin polymer and gel filtration on Biogel P-150 and proved to be electrophoretically homogeneous. Some of their physicochemical and biochemical properties are reported.