New cloning vectors with temperature-sensitive replication.

Research paper by G J GJ Phillips

Indexed on: 15 Jan '99Published on: 15 Jan '99Published in: Plasmid


A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restriction sites and provide for screening of recombinant plasmids by alpha complementation. These vectors have proven useful for a variety of applications where conditional replication of a recombinant plasmid is desired.