Natamycin and voriconazole exhibit synergistic interactions with non-antifungal ophthalmic agents against Fusarium species ocular isolates.

Research paper by Christiaan A CA Rees, Ruina R Bao, Michael E ME Zegans, Robert A RA Cramer

Indexed on: 20 Aug '19Published on: 24 Apr '19Published in: Antimicrobial agents and chemotherapy


The activities of two antifungal drugs in combination with four non-antifungal ophthalmic agents were evaluated using a micro-broth dilution method and a collection of eight ocular isolates that exhibited resistance to both natamycin (MICs: 14-32 μg/mL) and voriconazole (4->128 μg/mL). Synergistic interactions were observed for natamycin with 5-fluorouracil (FICIs: 0.34-0.61) and natamycin with timolol (0.41-0.52). Synergistic or indifferent interactions were observed for natamycin with EDTA (0.38-1.00), natamycin with dorzolamide (0.53-1.00), voriconazole with EDTA (0.38-1.00), voriconazole with timolol (0.39-0.94), and voriconazole with 5-fluorouracil (0.53-0.75). Taken together, these data suggest commonly used ophthalmic agents enhance the activity of antifungal drugs against drug recalcitrant ocular fungal pathogens. Copyright © 2019 American Society for Microbiology.