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Mutational analysis of Arabidopsis PP2CA2 involved in abscisic acid signal transduction.

Research paper by Xin-Hong XH Guo, Ke-Qin KQ Deng, Jie J Wang, Da-Shi DS Yu, Qiong Q Zhao, Xuan-Ming XM Liu

Indexed on: 07 Jul '09Published on: 07 Jul '09Published in: Molecular Biology Reports



Abstract

The homozygous T-DNA mutant of the PP2CA2 gene in Arabidopsis thaliana was identified at DNA and RNA levels. The semi-quantitative RT-PCR analysis showed expression of PP2CA2 was induced by NaCl and ABA. When grown in presence of increasing concentration of exogenous ABA the pp2ca2 mutant showed a significant loss of ABA sensitivity in terms of seed germination, efficiency of post-germination growth and root growth. In presence of all ABA and NaCl concentrations tested the germination percentage of wild-type seeds was lower than that of mutant ppca2 seeds. Furthermore, in the presence of exogenous ABA, the pp2ca2 seeds showed higher germination percentages than wild-type at different stages of development and the pp2ca2 seedlings showed a reduced inhibition of root growth compared with wild-type plants. The above results indicated that the pp2ca2 was an ABA-hyposensitive mutant.