Multi-Element (C, N, H, O) Stable Isotope Ratio Analysis for Determining the Geographical Origin of Pure Milk from Different Regions

Research paper by Donghui Luo, Hao Dong, Haiying Luo, Yanping Xian, Xindong Guo, Yuluan Wu

Indexed on: 05 Jun '15Published on: 05 Jun '15Published in: Food Analytical Methods


In order to identify the geographical origin of pure milk, different proteins and milk water were extracted from pure milk originated from Australia and New Zealand, Germany and France, the USA, and China. Then, δ13C and δ15N values of the extracted proteins and δ2H and δ18O values of milk water were determined by element analyzer-isotope ratio mass spectrometry (EA-IRMS). The results indicated that pure milk from these regions could be potentially discriminated by using analyte δ13C, δ15N, δ2H, and δ18O. The P value analysis of them was highly significant (P < 0.01) considering the origins. 3D distribution of δ13C, δ15N, δ2H, and δ18O can also clearly present regional concentration phenomena according to the regions of pure milk. Therefore, it can be concluded that δ13C, δ15N, δ2H, and δ18O analysis could potentially be rapid and routine for origin discrimination of pure milk.