Quantcast

Modes d'entrée des trophozoïtes deToxoplasma gondii dans les macrophages péritonéaux de souris normales et dans les cellules Hela en culture. Une étude microcinématographique en contraste de phase

Research paper by Bao Tho Nguyen, Simone Stadtsbaeder

Indexed on: 01 Jun '79Published on: 01 Jun '79Published in: Zeitschrift fur Parasitenkunde (Berlin, Germany)



Abstract

The mode of entry of living trophozoites ofToxoplasma gondii (RH strain) into normal mouse peritoneal macrophage and HeLa cell monolayers was studied by phase-contrast microcinematography. The results have shown thatToxoplasma can enter into macrophages either by phagocytosis (Figs. 1 and 2) and/or by active penetration (Fig. 3). Only the latter process was observed with normally non-phagocytic HeLa cells (Fig. 4). During this process the parasites actively moved towards the host-cells by flexion and penetrated them always through their sharpest end. Active penetration was a rapid phenomenon (about 20 s at 37°C) and was accompanied by a series of morphological changes, i.e., elongation of the anterior end, contraction and swelling of the parasite body. Contrasting with phagocytosis, toxoplasmas which had penetrated into the cell were not immediately isolated from the host-cytoplasm by a microscopically discernable vacuole. The nature of the process of penetration (pressure and/or perforation of the plasma membrane) is discussed.