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Methylation-specific PCR for detection of neoplastic DNA in biopsy washings.

Research paper by Carsten C Goessl, Markus M Müller, Rüdiger R Heicappell, Hans H Krause, Martin M Schostak, Bernd B Straub, Kurt K Miller

Indexed on: 22 Feb '02Published on: 22 Feb '02Published in: The Journal of Pathology



Abstract

Epigenetic DNA alterations such as promoter hypermethylation of glutathione S-transferase P1 (GSTP1) in prostatic adenocarcinoma frequently constitute tumour biomarkers. Neoplastic transformation was identified in washings of prostate biopsies by GSTP1 promoter hypermethylation, using methylation-specific PCR (MSP). Twenty-six patients undergoing sextant transrectal prostate biopsies for clinically suspected prostate cancer were enrolled. All ten patients diagnosed with adenocarcinoma (100%) and four of six patients with prostatic intraepithelial neoplasia (67%), but none of ten patients with benign hyperplasia (0%), exhibited GSTP1 promoter hypermethylation in at least one out of six biopsy washings. Since this approach is transferable to various cancer entities, a sensitive and specific DNA-based analysis of biopsy material seems generally feasible without impeding routine histopathological examination.

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