Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes.

Research paper by Leifu L Chang, David D Barford

Indexed on: 23 Sep '18Published on: 23 Sep '18Published in: Methods in molecular biology (Clifton, N.J.)


The recent resolution revolution in cryo-electron microscopy has generated a huge interest in the technique for determining atomic resolution structures of large and dynamic macromolecular complexes that are intractable to crystallography and NMR. A key element of success in cryo-EM is the quality of the specimen vitrified on the cryo-EM grid. In this chapter we outline methods for cryo-EM grid sample preparation.