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M-CSF targeting into LCL nucleus behaves as a malignancy promotor

Research paper by Zhen-yu Cao, Ke-fu Wu, Yu-hua Song, Ge Li, Yong-min Lin, Qing Rao, Xiao-tong Ma

Indexed on: 01 Dec '03Published on: 01 Dec '03Published in: Chinese Journal of Cancer Research



Abstract

Objective: To investigate the functions of nM-CSF in malignant cells. Methods: recombinant M-CSF was targeted into cell nucleus by employing a eukaryotic expression plasmid vector pCMV/myc/nuc. The constructed plasmid was transfected into cells of EBV transformed lymphoblastoid cell line (LCL). RT-PCR, Western blot and immunofluorescent staining showed that recombinant M-CSF was localized into LCL cell nucleus. The transgenic cells showed elevated proliferation potential, enhanced resistance to apoptosis and increased ability ofin vitro migration. Conclusion: Nucleus presenting M-CSF might act as a promoting factor in the processes of cell malignancy.