Loss of arbuscular mycorrhizal fungal diversity in trap cultures during long-term subculturing.

Research paper by Dora D Trejo-Aguilar, Liliana L Lara-Capistrán, Ignacio E IE Maldonado-Mendoza, Ramón R Zulueta-Rodríguez, Wendy W Sangabriel-Conde, María Elena ME Mancera-López, Simoneta S Negrete-Yankelevich, Isabelle I Barois

Indexed on: 25 Feb '14Published on: 25 Feb '14Published in: IMA fungus


Long-term successional dynamics of an inoculum of arbuscular mycorrhizal fungi (AMF) associated with the maize rhizosphere (from traditionally managed agroecosystems in Los Tuxtlas, Veracruz, Mexico), was followed in Bracchiaria comata trap cultures for almost eight years. The results indicate that AMF diversity is lost following long-term subculturing of a single plant host species. Only the dominant species, Claroideoglomus etunicatum, persisted in pot cultures after 13 cycles. The absence of other morphotypes was demonstrated by an 18S rDNA survey, which confirmed that the sequences present solely belonged to C. etunicatum. Members of Diversisporales were the first to decrease in diversity, and the most persistent species belonged to Glomerales.