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LncRNA XIST enhanced TGF-β2 expression by targeting miR-141-3p to promote pancreatic cancer cells invasion.

Research paper by Jianmin J Sun, Yubao Y Zhang

Indexed on: 21 Jun '19Published on: 20 Jun '19Published in: Bioscience Reports



Abstract

The level expression of LncRNA XIST is up-regulated in pancreatic cancer. However, the role of XIST in pancreatic cancer and the underlying mechanism are still unknown. The present study aimed to elucidate how XIST participates in pancreatic cancer and its potential target, miR-141-3p. We detected the XIST expression in pancreatic cancer tissues and cells by qRT-PCR. Cell proliferation was measured using a CCK8 kit, and the migration and invasion of cells was measured by Transwell assay. Silencing XIST and miR-141-3p was performed with transfection by Lipofectamine kit. Binding assay was conducted by luciferase reporter assay. Protein expression was examined by western blot. These results indicate that 1)XIST is highly expressed in tumor tissues while miR-141-3p is down-regulated. 2)Silencing XIST inhibits the pancreatic cell proliferation, migration and invasion. 3)MiR-141-3p inhibitor alleviates the inhibitory effect by siXIST in pancreatic cancer cell lines. 4)MiR-141-3p directly interacts with XIST and also negatively regulates TGF-β2 expression. 5)Overexpression of XIST attenuates the inhibition of TGF-β2 expression by miR-141-3p. The conclusion,is that XIST could promote proliferation, migration and invasion of pancreatic cancer cells via miR-141-5p/ TGF-β2 axis. ©2019 The Author(s).