Quantcast

Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry.

Research paper by Susanne S Sales, Oskar O Knittelfelder, Andrej A Shevchenko

Indexed on: 05 Jul '17Published on: 05 Jul '17Published in: Methods in molecular biology (Clifton, N.J.)



Abstract

Clinical lipidomics is an emerging biomarker discovery approach that compares lipid profiles under pathologically and physiologically normal conditions. Here we describe a method for the absolute (molar) quantification of more than 200 molecules from 14 major lipid classes from 5 μL of human blood plasma using high-resolution top-down shotgun mass spectrometry. Because of its technical simplicity and robustness, the protocol lends itself for high-throughput clinical lipidomics screens.