Indexed on: 16 Feb '10Published on: 16 Feb '10Published in: Journal of Separation Science
Different hydrophilic interaction chromatography (HILIC) columns were screened for lactic acid separation in hand dishwashing liquid products and the influence of mobile phase strength, buffer concentration and column temperature on the retention of lactic acid on a Zorbax NH(2) column was investigated. An isocratic HILIC method for the quantitation of lactic acid in hand dishwashing liquid products was developed. The mobile phase consists of 70% methanol and 30% 20 mM sodium phosphate buffer (v/v) at pH 2.5. The HILIC stationary phase is Zorbax NH(2), 250x4.6 with a 5 microm particle size. Detection was carried out using a variable wavelength UV-VIS detector at 226 nm. The linear range and percent recovery for lactic acid in the products were 44.68-1206.39 microg/mL and 100.3%, respectively. This paper provides an optimized HILIC methodology for the analysis of an acidic polar analyte (lactic acid) on a basic stationary phase. The proposed method can be used for the routine analysis of lactic acid.