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Knockdown of ezrin suppresses the migration and angiogenesis of human umbilical vein endothelial cells in vitro

Research paper by Liang-ping Zhao赵良平, Lei Huang黄 磊, Xun Tian田 训, Feng-qi Liang梁逢奇, Jun-cheng Wei魏军成, Xian Zhang张 娴, Sha Li李 莎, Qing-hua Zhang张庆华

Indexed on: 13 Apr '16Published on: 13 Apr '16Published in: Journal of Huazhong University of Science and Technology [Medical Sciences]



Abstract

Progressive tumor growth is dependent on angiogenesis. The mechanisms by which endothelial cells (ECs) are incorporated to develop new blood vessels are not well understood. Recent studies reveal that the ezrin radixin moesin (ERM) family members are key regulators of cellular activities such as adhesion, morphogenetic change, and migration. We hypothesized that ezrin, one of the ERM family members, may play important roles in ECs organization during angiogenesis, and new vessels formation in preexisting tissues. To test this hypothesis, in this study, we investigated the effects of ezrin gene silencing on the migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro. HUVECs were transfected with plasmids with ezrin-targeting short hairpin RNA by using the lipofectamine-2000 system. Wound assay in vitro and three-dimensional culture were used to detect the migration and angiogenesis capacity of HUVECs. The morphological changes of transfected cells were observed by confocal and phase contrast microscopy. Our results demonstrated that the decreased expression of ezrin in HUVECs significantly induced the morphogenetic changes and cytoskeletal reorganization of the transfected cells, and also reduced cell migration and angiogenesis capacity in vitro, suggesting that ezrin play an important role in the process of HUVECs migration and angiogenesis.