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Isolation of thermostable D-hydantoinase-producing thermophilicBacillus sp SD-1

Research paper by Seung-Goo Lee, Dong-Cheol Lee, Moon-Hee Sung, Hak-Sung Kim

Indexed on: 01 May '94Published on: 01 May '94Published in: Biotechnology Letters



Abstract

A thermophilic bacterium which showed highest thermostability and activity of the hydantoinase was isolated from 1m000 thermophiles and identified to beBacillus sp. SD-1 according to morphological and physiological characteristics. The optimal growth temperature of the bacterium was about 60°C. The hydantoinase ofBacillus sp. SD-1 was strictly D-specific, and optimal pH and temperature were determined to be about 8.0 and 70°C, respectively. The D-hydantoinase was stable upto 70°C, and half-life of the enzyme was about 20 min at 80°C.