Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart.

Research paper by Yukiteru Y Ouji, Masahide M Yoshikawa, Kei K Moriya, Mariko M Nishiofuku, Noriko N Ouji-Sageshima, Ryosuke R Matsuda, Fumihiko F Nishimura, Shigeaki S Ishizaka

Indexed on: 16 Jun '10Published on: 16 Jun '10Published in: Journal of bioscience and bioengineering


A method for obtaining mouse hepatocytes by infusing collagenase solution into the left ventricle was established. This technique was shown to be equivalent to the intra-portal infusion method and more practical, especially in postnatal mice with a small body size.