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Interlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.

Research paper by Marcus S MS Cooke, Lars L Barregard, Vilas V Mistry, Neelam N Potdar, Rafal R Rozalski, Daniel D Gackowski, Agnieszka A Siomek, Marek M Foksinski, Peter P Svoboda, Hiroshi H Kasai, Justin C JC Konje, Gerd G Sallsten, Mark D MD Evans, Ryszard R Olinski

Indexed on: 31 Mar '09Published on: 31 Mar '09Published in: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals



Abstract

Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Here we report the comparison of two, distinct chromatographic assays with an enzyme-linked immunosorbent assay (ELISA). The chromatographic assays displayed good agreement (r =:0.89, p < 0.0001), whereas there was markedly worse, albeit still significant, agreement with the ELISA (high-pressure liquid chromatography followed by gas chromatography (HPLC-GC/MS), r = 0.43; HPLC with electrochemical detection (HPLC-EC), r = 0.56; p < 0.0001). Mean values differed significantly between the chromatographic assays and the ELISA (HPLC-GC/MS 3.86, HPLC-EC 4.20, ELISA 18.70 ng mg(-1) creatinine; p < 0.0001). While it is reassuring to note good agreement between chromatographic assays, this study reveals significant short-comings in the ELISA, which brings into question its continued use in its present form.