Influence of eugenol-based materials and cleaning procedures previously to resin composite restoration on microleakage after 1-year storage

Research paper by Murilo Baena Lopes, Klissia Romero Felizardo, Renan Hideki Kaneshima, Sandrine Berger Guiraldo, Ricardo Danil Guiraldo, Alcides Gonini Júnior

Indexed on: 12 Jul '16Published on: 12 Jul '16Published in: Applied Adhesion Science


The interference of eugenol on the adhesive systems and composite polymerization could promote a higher marginal microleakage. This could be reduced by mechanical and chemical cleansing. The objective of this study was to evaluate the influence of the previous use of eugenol-based materials to a permanent restoration on the marginal microleakage. The influence of mechanical cleansing associated with chemical cleansing with chlorhexidine gel was also evaluated. Non-eugenol endodontic sealer and temporary material and mechanical cleansing was used as a control. Eighty bovine incisors were endodontic treated and divided into eight groups (n = 10) according to the sealer, temporary material and removal method. The specimens were temporarily restored and stored for 1 week at 37 °C and 100 % relative humidity, followed by definitive restoration and one-year storage at the same environment. The teeth were then sealed, immersed in silver nitrate at 50 % for 2 h and sectioned to have the microleakage qualitatively and quantitatively visually analyzed under a stereomicroscope at 45×. The percentage data were submitted to an ANOVA and Tukey’s test, and the scores were submitted to a Kruskal–Wallis and Dunn’s test at 5 % of the significance level. There was no difference between the groups when mechanical removal was made (p > 0.05). When chlorhexidine was used, the group that used endodontic eugenol based material as a sealer and the resin-based material as a temporary restoration showed less microleakage than the other (p < 0.05). All groups showed less microleakage when chlorhexidine was used instead of solely using mechanical removal (p < 0.05). For scores, when the eugenol based sealer was combined with resin-based temporary material, and when the resin sealer was combined with eugenol based temporary material using chlorhexidine, a lower median was found (p < 0.05) compared to all other groups. Eugenol showed no influence, and chlorhexidine gel showed to be effective in reducing microleakage.